Structural and Functional Characterization of O-Antigen Translocation and Polymerization in Pseudomonas aeruginosa PAO1

Structural and Functional Characterization of O-Antigen Translocation and Polymerization in Pseudomonas aeruginosa PAO1

Heteropolymeric O antigen (O-Ag)-capped lipopolysaccharide is the principal constituent of the Gram-negative bacterial cell surface. It is assembled via the integral inner membrane (IM) Wzx/Wzy-dependent pathway. In Pseudomonas aeruginosa, Wzx translocates lipid-linked anionic O-Ag subunits from t...

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Bibliographic Details
Main Author: Islam, Salim Timo
Other Authors: Lam, Joseph
Language:en
Published: 2013
Subjects:
lipopolysaccharide
flippase
polymerase
chain-length regulator
polysaccharide co-polymerase
O antigen
Wzx/Wzy-dependent
membrane protein
topology mapping
C-terminal reporter
iodide efflux
iodide flux
anion flux
proteoliposome
Wzx
Wzy
Wzz
Wzz1
Wzz2
site-directed mutagenesis
protein purification
detergent solubilization
vesicle reconstitution
alkaline phosphatase
beta-galactosidase
normalized activity ratio
antiport
GFP
topology prediction
homology model
Western immunoblot
silver stain
structure prediction
proton-dependent gating
coupling ion
proton uptake
catch-and-release mechanism
arginine
remote homologue detection
biochemistry
biophysics
microbiology
structural biology
genetics
bioinformatics
Online Access:http://hdl.handle.net/10214/7239

Internet

http://hdl.handle.net/10214/7239

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