Summary: | In the first experiment, two multiparous and two primiparous Holstein cows in midlactation were randomly assigned to four diets in a 4 x 4 Latin square design. Diets consisted of total mixed ration (TMR) plus (i) 0% added fat-Control, (ii) Linola, a low linolenic (C18:3), high linoleic (C18:2) variety of Solin at 400-450 9 fat per kg milk fat produced, (iii) flaxseed at 200-225 g fat per kg milk fat produced (FL) and (iv) flaxseed at 400-450 g fat per kg milk fat produced (FH). The high level of supplemental fat provided approximately 123 g C18:3 to the milk fat, representing a theoretical value of 10% C18:3, assuming an average production of 36 kg milk with 3.4% fat. In the second experiment, four Holstein primiparous cows in midlactation were randomly assigned to a 4 x 4 Latin square. Diets consisted of TMR top dressed with (i) 0% added fat, Control, (ii) formaldehyde treated flaxseed (FH) at 1.64 kg, (iii) low level lignosulfonate treated flaxseed at 0.57 kg (LL) and (iv) high level of lignosulfonate treated flaxseed at 1.14 kg (LH). Approximately 377 g fat, 193 g fat and 386 g of fat were provided by the top dress. The flaxseed was treated with lignosulfonate by EXL Milling (Hassall, Sask.), ground and heated to 155$\sp\circ$C and steeped for 30 minutes at a beginning temperature of 124$\sp\circ$C and an end temperature of 118$\sp\circ$C. (Abstract shortened by UMI.)
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