Fluorescein, acrylodan and pyrene conjugates of horse plasma gelsolin : responses to calcium, actin and tropomyosin
Gelsolin, a protein involved in the regulation of actin filament length, was isolated from horse plasma and modified with the amine-selective reagent fluorescein-5-isothiocyanate (FITC). The extent of FITC incorporation per gelsolin molecule was 4.8 ± 0.6, based upon 4 labelling trials. FITC-gels...
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| Language: | English |
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2008
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| Online Access: | http://hdl.handle.net/2429/3066 |