Observation and catalytic promiscuity of retaining glycosidase intermediates
This thesis is divided into two parts. The first addresses basic issues of retaining glycosidase mechanism. This includes the use of a new technique, time-resolved electrospray mass spectrometry, to directly monitor changes in the mass of Bacillus circulans |3-xylanase during its catalytic cycle....
| Main Author: | |
|---|---|
| Language: | English |
| Published: |
2009
|
| Online Access: | http://hdl.handle.net/2429/13892 |