Preparation of a Flavonol Specific Glucosyltransferase found in Grapefruit and Site-Directed Mutants for Protein Crystallization

This research was designed to determine the conditions necessary to remove c-myc and 6x-His tags from a flavonol specific glucosyltransferase found in grapefruit (CP3GT) using thrombin in preparation for crystallization. X-ray crystallography of CP3GT crystals may elucidate structural features that...

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Bibliographic Details
Main Author: Birchfield, Aaron
Format: Others
Language:English
Published: Digital Commons @ East Tennessee State University 2019
Subjects:
Online Access:https://dc.etsu.edu/etd/3523
https://dc.etsu.edu/cgi/viewcontent.cgi?article=4981&context=etd