A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate

A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate

Elevated reaction temperatures are crucial for the efficient enzymatic degradation of polyethylene terephthalate (PET). A disulfide bridge was introduced to the polyester hydrolase TfCut2 to substitute its calcium binding site. The melting point of the resulting variant increased to 94.7°C (wild-typ...

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Bibliographic Details
Main Authors: Then, Johannes, Wei, Ren, Oeser, Thorsten, Gerdts, André, Schmidt, Juliane, Barth, Markus, Zimmermann, Wolfgang
Other Authors: Universität Leipzig, Fakultät für Biowissenschaften, Pharmazie und Psychologie
Format: Article
Language:English
Published: Universitätsbibliothek Leipzig 2016
Subjects:
Biokatalyse
Kalzium
Disulfidbrücke
Polyethylenterephthalat
Protein-Engineering
Proteinstabilität
biocatalysis
calcium
disulfide bridge
polyethylene terephthalate
protein engineering
protein stability
ddc:540
ddc:570
Online Access:http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205582
http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205582
http://www.qucosa.de/fileadmin/data/qucosa/documents/20558/OAP-2016-090_Zimmermann_feb412053.pdf

Internet

http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205582
http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205582
http://www.qucosa.de/fileadmin/data/qucosa/documents/20558/OAP-2016-090_Zimmermann_feb412053.pdf

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