Stepwise error-prone PCR and DNA shuffling changed the pH activity
range and product specificity of the cyclodextrin glucanotransferase
from an alkaliphilic Bacillus sp.

Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.

Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilic Bacillus sp. G-825-6 converts starch mainly to c-cyclodextrin (CD8). A combination of error-prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product specificity for CD8 and a broad pH activity range...

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Bibliographic Details
Main Authors:	Melzer, Susanne, Sonnendecker, Christian, Föllner, Christina, Zimmermann, Wolfgang
Other Authors:	Universität Leipzig, Institut für Biochemie
Format:	Article
Language:	English
Published:	Universitätsbibliothek Leipzig 2015
Subjects:	Cyclodextrin-Glucosyl-Transferase Bacillus sp. Gamma-Cyclodextrin zufällige Mutagenese DNA-Shuffling Cyclodextrin glucanotransferase Gamma-cyclodextrin Random mutagenesis DNA shuffling ddc:572
Online Access:	http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-172353 http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-172353 http://www.qucosa.de/fileadmin/data/qucosa/documents/17235/OAP-2015-104-Zimmermann_1-s2.0-S221154631500056X-main.pdf

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