Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.

Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.

Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilic Bacillus sp. G-825-6 converts starch mainly to c-cyclodextrin (CD8). A combination of error-prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product specificity for CD8 and a broad pH activity range...

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Bibliographic Details
Main Authors: Melzer, Susanne, Sonnendecker, Christian, Föllner, Christina, Zimmermann, Wolfgang
Other Authors: Universität Leipzig, Institut für Biochemie
Format: Article
Language:English
Published: Universitätsbibliothek Leipzig 2015
Subjects:
Cyclodextrin-Glucosyl-Transferase
Bacillus sp.
Gamma-Cyclodextrin
zufällige Mutagenese
DNA-Shuffling
Cyclodextrin glucanotransferase
Gamma-cyclodextrin
Random mutagenesis
DNA shuffling
ddc:572
Online Access:http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-172353
http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-172353
http://www.qucosa.de/fileadmin/data/qucosa/documents/17235/OAP-2015-104-Zimmermann_1-s2.0-S221154631500056X-main.pdf

Internet

http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-172353
http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-172353
http://www.qucosa.de/fileadmin/data/qucosa/documents/17235/OAP-2015-104-Zimmermann_1-s2.0-S221154631500056X-main.pdf

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