Dihydrofolate reductase gene amplification in human cell lines VA2-B and hela BU25
Mammalian gene amplification has been studied using as a model system the amplification of the endogenous Dihydrofolate Reductase (DHFR) gene in the human cell lines VA[subscript 2]-B and Hela BU25. Cell lines were stepwise-selected to high DHFR gene copy number using methotrexate, a DHFR enzyme inh...