The Expression, Stability, and Enzymatic Activity of Specific Beta-Lactamase Mutants

The Expression, Stability, and Enzymatic Activity of Specific Beta-Lactamase Mutants

<p>Three catalytic site mutants (ser 70 to thr, thr 71 to ser, and the double mutant ser 70 to thr-thr 71 to ser) of the pBR 322 beta-lactamase were purified to homogeneity. These mutant proteins were unstable and could only be obtained from <i>E. coli</i> at 30°C, rather than the...

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Bibliographic Details
Main Author: Neitzel, James Joseph
Format: Others
Published: 1987
Online Access:https://thesis.library.caltech.edu/11471/2/Neitzel_JJ_1987.pdf
Neitzel, James Joseph (1987) The Expression, Stability, and Enzymatic Activity of Specific Beta-Lactamase Mutants. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/E6R6-4J57. https://resolver.caltech.edu/CaltechTHESIS:04162019-152444688 <https://resolver.caltech.edu/CaltechTHESIS:04162019-152444688>

Internet

https://thesis.library.caltech.edu/11471/2/Neitzel_JJ_1987.pdf
Neitzel, James Joseph (1987) The Expression, Stability, and Enzymatic Activity of Specific Beta-Lactamase Mutants. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/E6R6-4J57. https://resolver.caltech.edu/CaltechTHESIS:04162019-152444688 <https://resolver.caltech.edu/CaltechTHESIS:04162019-152444688>

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