Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

Chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherich...

Full description

Bibliographic Details
Main Authors: Baker, David (Author), Ting, Alice Y. (Contributor), Liu, Daniel S. (Contributor), Nivon, Lucas G. (Author), Richter, Florian (Author), Goldman, Peter J. (Contributor), Yao, Jennifer Z. (Contributor), Phipps, William S. (Contributor), Ye, Anne Z. (Contributor), Deerinck, Thomas J. (Author), Richardson, Douglas (Author), Ellisman, Mark H. (Author), Drennan, Catherine L (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor), Drennan, Catherine L. (Contributor)
Format: Article
Language:English
Published: National Academy of Sciences (U.S.), 2015-04-01T15:48:59Z.
Subjects:
Article
Online Access:Get fulltext

Internet

Get fulltext

Similar Items