Optimized fluorescent labeling to identify memory B cells specific for Neisseria meningitidis serogroup B vaccine antigens ex vivo

Optimized fluorescent labeling to identify memory B cells specific for Neisseria meningitidis serogroup B vaccine antigens ex vivo

Antigen-specific memory B cells generate anamnestic responses and high affinity antibodies upon re-exposure to pathogens. Attempts to isolate rare antigen-specific memory B cells for in-depth functional analysis at the single-cell level have been hindered by the lack of tools with adequate sensitivi...

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Main Authors: Nair, Nitya (Author), Buti, Ludovico (Author), Faenzi, Elisa (Author), Buricchi, Francesca (Author), Nuti, Sandra (Author), Sammicheli, Chiara (Author), Tavarini, Simona (Author), Popp, Maximilian W.L (Contributor), Ploegh, Hidde (Contributor), Berti, Francesco (Author), Pizza, Mariagrazia (Author), Castellino, Flora (Author), Finco, Oretta (Author), Rappuoli, Rino (Author), Del Giudice, Giuseppe (Author), Galli, Grazia (Author), Bardelli, Monia (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Whitehead Institute for Biomedical Research (Contributor)
Format: Article
Language:English
Published: Wiley Blackwell, 2014-12-24T20:20:19Z.
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