Imaging Protein-Protein Interactions inside Living Cells via Interaction-Dependent Fluorophore Ligation

Imaging Protein-Protein Interactions inside Living Cells via Interaction-Dependent Fluorophore Ligation

We report a new method, Interaction-Dependent PRobe Incorporation Mediated by Enzymes, or ID-PRIME, for imaging protein-protein interactions (PPIs) inside living cells. ID-PRIME utilizes a mutant of Escherichia coli lipoic acid ligase, LplA[superscript W37V], which can catalyze the covalent ligation...

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Bibliographic Details
Main Authors: Slavoff, Sarah A. (Contributor), Liu, Daniel S. (Contributor), Cohen, Justin D. (Contributor), Ting, Alice Y. (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Chemistry (Contributor)
Format: Article
Language:English
Published: American Chemical Society (ACS), 2013-11-22T19:46:40Z.
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