Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human plu...

Full description

Bibliographic Details
Main Authors: Saha, Krishanu (Author), Mei, Ying (Contributor), Reisterer, Colin M. (Contributor), Pyzocha, Neena Kenton (Author), Yang, Jing (Contributor), Muffat, Julien (Author), Davies, Martyn C. (Contributor), Alexander, Morgan R. (Contributor), Jaenisch, Rudolf (Contributor), Langer, Robert S (Author), Anderson, Daniel Griffith (Author)
Other Authors: Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor), Langer, Robert (Contributor), Anderson, Daniel G. (Contributor)
Format: Article
Language:English
Published: National Academy of Sciences, 2012-10-18T18:11:09Z.
Subjects:
Article
Online Access:Get fulltext

Internet

Get fulltext

Similar Items