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69920 |
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|a Love, Kerry Routenberg
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|a Massachusetts Institute of Technology. Department of Chemical Engineering
|e contributor
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|a Love, J. Christopher
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|a Love, J. Christopher
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|a Panagiotou, Vasiliki
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|a Love, Kerry Routenberg
|e contributor
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|a Panagiotou, Vasiliki
|e author
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|a Jiang, Bo
|e author
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|a Stadheim, Terrance A.
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|a Love, J. Christopher
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|a Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.
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|b John Wiley & Sons, Inc.,
|c 2012-04-04T14:56:52Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/69920
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|a The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner.
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|a Merck Research Laboratories
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|a en_US
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|a Article
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|t Biotechnology and Bioengineering
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