Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.
The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that con...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
John Wiley & Sons, Inc.,
2012-04-04T14:56:52Z.
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| Subjects: | |
| Online Access: | Get fulltext |