Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.

• Main Authors: Love, Kerry Routenberg (Contributor), Panagiotou, Vasiliki (Contributor), Jiang, Bo (Author), Stadheim, Terrance A. (Author), Love, J. Christopher (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor)
• Format: Article
• Language: English
• Published: John Wiley & Sons, Inc., 2012-04-04T14:56:52Z.
• Subjects: Article
• Online Access: Get fulltext

The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner.
Merck Research Laboratories