Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2

Synthetic methylotrophy, the modification of organisms such as E. coli to grow on methanol, is a longstanding goal of metabolic engineering and synthetic biology. The poor kinetic properties of NAD-dependent methanol dehydrogenase, the first enzyme in most methanol assimilation pathways, limit pathw...

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Bibliographic Details
Main Authors: Roth, Timothy B. (Author), Woolston, Benjamin Michael (Author), Stephanopoulos, Gregory (Author), Liu, David R. (Author)
Other Authors: Broad Institute of MIT and Harvard (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor)
Format: Article
Language:English
Published: American Chemical Society (ACS), 2020-07-30T22:10:00Z.
Subjects:
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