Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

In vivo, proteins are often part of large macromolecular complexes where binding specificity and dynamics ultimately dictate functional outputs. In this work, the pre-endosomal anthrax toxin is assembled and transitioned into the endosomal complex. First, the N-terminal domain of a cysteine mutant l...

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Bibliographic Details
Main Authors: Machen, Alexandra J. (Author), O'Neil, Pierce T. (Author), Pentelute, Bradley L. (Author), Villar, Maria T. (Author), Artigues, Antonio (Author), Fisher, Mark T. (Author)
Other Authors: Massachusetts Institute of Technology. Department of Chemistry (Contributor)
Format: Article
Language:English
Published: MyJove Corporation, 2020-01-24T16:21:47Z.
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