Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
In vivo, proteins are often part of large macromolecular complexes where binding specificity and dynamics ultimately dictate functional outputs. In this work, the pre-endosomal anthrax toxin is assembled and transitioned into the endosomal complex. First, the N-terminal domain of a cysteine mutant l...
Main Authors: | , , , , , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
MyJove Corporation,
2020-01-24T16:21:47Z.
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Subjects: | |
Online Access: | Get fulltext |