Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design
Large synthetic genetic circuits require the simultaneous expression of many regulators. Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) direct it to bind a promoter. The programmability and specificity of RNA:DNA basepairing simplifies the generation of many or...
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| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press,
2019-03-19T12:29:57Z.
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| Subjects: | |
| Online Access: | Get fulltext |