6 Principles and applications of temporal-focusing wide-field two-photon microscopy
Temporal focusing allows for rapid optically sectioned two-photon widefield microscopy. Depth sectioning is provided in a wide-field manner, without spatial focusing, by controlling the temporalwidth of femtosecond laser pulses near the focal plane. This spatial control of the temporal pulse width i...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
2019-01-04T18:38:14Z.
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Subjects: | |
Online Access: | Get fulltext |