Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

Genetic screens help infer gene function in mammalian cells, but it has remained difficult to assay complex phenotypes-such as transcriptional profiles-at scale. Here, we develop Perturb-seq, combining single-cell RNA sequencing (RNA-seq) and clustered regularly interspaced short palindromic repeats...

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Bibliographic Details
Main Authors: Dixit, Atray (Author), Parnas, Oren (Author), Li, Biyu (Author), Chen, Jenny (Author), Fulco, Charles P. (Author), Jerby-Arnon, Livnat (Author), Marjanovic, Nemanja D. (Author), Dionne, Danielle (Author), Burks, Tyler (Author), Raychowdhury, Raktima (Author), Adamson, Britt (Author), Norman, Thomas M. (Author), Weissman, Jonathan S. (Author), Friedman, Nir (Author), Lander, Eric Steven (Contributor), Regev, Aviv (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor)
Format: Article
Language:English
Published: Elsevier BV, 2018-06-29T19:01:58Z.
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