Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

The simplicity of programming the CRISPR (clustered regularly interspaced short palindromic repeats)-associated nuclease Cas9 to modify specific genomic loci suggests a new way to interrogate gene function on a genome-wide scale. We show that lentiviral delivery of a genome-scale CRISPR-Cas9 knockou...

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Bibliographic Details
Main Authors: Hartenian, E. (Author), Shi, X. (Author), Mikkelsen, T. S. (Author), Heckl, D. (Author), Ebert, B. L. (Author), Root, D. E. (Author), Doench, J. G. (Author), Shalem, Ophir (Contributor), Sanjana, Neville E (Contributor), Scott, David Arthur (Contributor), Zhang, Feng (Contributor)
Other Authors: McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS), 2017-09-15T20:18:35Z.
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