Directed evolution of APEX2 for electron microscopy and proximity labeling

Directed evolution of APEX2 for electron microscopy and proximity labeling

APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is fa...

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Bibliographic Details
Main Authors: Kamer, Kimberli J (Author), Deerinck, Thomas J (Author), Ellisman, Mark H (Author), Mootha, Vamsi K (Author), Lam, Stephanie Shih-Min (Contributor), Martell, Jeffrey Daniel (Contributor), Ting, Alice Y (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Chemistry (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2017-07-11T13:04:21Z.
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