Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

Show other versions (1)

In recent years, the assay for transposase-accessible chromatin using sequencing (ATAC-Seq) has become a fundamental tool of epigenomic research. However, it is difficult to perform this technique on frozen samples because freezing cells before extracting nuclei can impair nuclear integrity and alte...

Full description

Bibliographic Details
Main Authors: Shelley, Brandon C. (Author), Mandefro, Berhan (Author), Sareen, Dhruv (Author), Svendsen, Clive N. (Author), Milani, Pamela (Contributor), Escalante, Renan A. (Contributor), Patel-Murray, Natasha Leanna (Contributor), Xin, Xiaofeng (Contributor), Adam, Miriam (Contributor), Fraenkel, Ernest (Contributor)
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2017-05-26T23:40:28Z.
Subjects:
Article
Online Access:Get fulltext

Internet

Get fulltext

Similar Items