Single Guide RNA Library Design and Construction
This protocol describes how to generate a single guide RNA (sgRNA) library for use in genetic screens. There are many online tools available for predicting sgRNA sequences with high target specificity and/or cleavage activity. Here, we refer the user to genome-wide sgRNA sequence predictions that we...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Cold Spring Harbor Laboratory Press,
2016-12-09T17:11:51Z.
|
| Subjects: | |
| Online Access: | Get fulltext |