Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2
This protocol describes a method to obtain spatially resolved proteomic maps of specific compartments within living mammalian cells. An engineered peroxidase, APEX2, is genetically targeted to a cellular region of interest. Upon the addition of hydrogen peroxide for 1 min to cells preloaded with a b...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group,
2016-08-29T15:28:53Z.
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| Subjects: | |
| Online Access: | Get fulltext |