Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2

Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2

This protocol describes a method to obtain spatially resolved proteomic maps of specific compartments within living mammalian cells. An engineered peroxidase, APEX2, is genetically targeted to a cellular region of interest. Upon the addition of hydrogen peroxide for 1 min to cells preloaded with a b...

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Bibliographic Details
Main Authors: Hung, Victoria (Contributor), Udeshi, Namrata D. (Author), Lam, Stephanie Shih-Min (Contributor), Loh, Ken H. (Contributor), Cox, Kurt James (Contributor), Pedram, Kayvon (Contributor), Carr, Steven A. (Author), Ting, Alice Y. (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Chemistry (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2016-08-29T15:28:53Z.
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