Cas9 Functionally Opens Chromatin

Cas9 Functionally Opens Chromatin

Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at prev...

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Bibliographic Details
Main Authors: Barkal, Amira A. (Contributor), Srinivasan, Sharanya (Author), Gifford, David K. (Contributor), Sherwood, Richard I. (Author), Hashimoto, Tatsunori Benjamin (Contributor)
Other Authors: Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor)
Format: Article
Language:English
Published: Public Library of Science, 2016-05-23T14:44:15Z.
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