Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active St...

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Bibliographic Details
Main Authors: Joung, Julia (Contributor), Zhang, Feng (Contributor), Konermann, Silvana (Contributor), Dahlman, James E. (Author), Abudayyeh, Omar Osama (Contributor), Gootenberg, Jonathan S. (Contributor)
Other Authors: Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2016-05-22T23:41:33Z.
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