Combination of oxyanion Gln114 Mutation and medium engineering to influence the enantioselectivity of thermophilic lipase from geobacillus zalihae
The substitution of the oxyanion Q114 with Met and Leu was carried out to investigate the role of Q114 in imparting enantioselectivity on T1 lipase. The mutation improved enantioselectivity in Q114M over the wild-type, while enantioselectivity in Q114L was reduced. The enantioselectivity of the ther...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG,
2012-09.
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| Subjects: | |
| Online Access: | Get fulltext |