Overexpression, purification and characterization of Aspergillus niger beta-glucosidase in Pichia pastoris

This study describes the expression of β-glucosidase (BglA) from Aspergillus niger in Pichia pastoris, a methylotrophic yeast strain, under the regulation of an alcohol oxidase promoter. The heterologous expression of BglA was optimized in a shake flask. Optimal conditions were achieved using an ini...

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Bibliographic Details
Main Authors: Kamaruddin, S. (Author), Abu Bakar, F.D (Author), Illias, R.M (Author), Said, M. (Author), Hassan, O. (Author), Murad, A.M.A (Author)
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia, 2015-04.
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