Overexpression, purification and characterization of Aspergillus niger beta-glucosidase in Pichia pastoris
This study describes the expression of β-glucosidase (BglA) from Aspergillus niger in Pichia pastoris, a methylotrophic yeast strain, under the regulation of an alcohol oxidase promoter. The heterologous expression of BglA was optimized in a shake flask. Optimal conditions were achieved using an ini...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Penerbit Universiti Kebangsaan Malaysia,
2015-04.
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Online Access: | Get fulltext |