Assessment of critical parameters in blood processing for the bovine interferon-gamma ELISPOT assay to detect Mycobacterium bovis infected cattle in India

Assessment of critical parameters in blood processing for the bovine interferon-gamma ELISPOT assay to detect Mycobacterium bovis infected cattle in India

In vitro production of bovine interferon gamma (BoIFN-γ) cyokine from bovine peripheral blood mononuclear cells (PBMCs) can be detected using the most sensitive enzyme-linked immunosorbent spot (ELISPOT) assay. ELISPOT assays are dependent on the quantity and quality of PBMC preparations and hence c...

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Bibliographic Details
Main Authors: Maroudam Veerasami, Loganathan Moorthy, Dipankar Das, Sugumar Parthasarath, Dev Chandran, Rajendra Lingal, Villuppanoor Alwar Srinivasan
Format: Article
Language:English
Published: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale 2011-01-01
Series:Veterinaria Italiana
Subjects:
Bovine tuberculosis
ELISPOT assay
Enzyme-linked immunosorbent spot
IFN-γ
India
Interferon gamma
Mycobacterium bovis
Peripheral blood mononuclear cells
Tuberculosis.
Online Access:http://www.izs.it/vet_italiana/2011/47_1/25.pdf

Internet

http://www.izs.it/vet_italiana/2011/47_1/25.pdf

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