Rectification of artificial molecular recombination with the use of high fidelity enzyme in the amplification of 16S rDNA sequences from Stool sample

Rectification of artificial molecular recombination with the use of high fidelity enzyme in the amplification of 16S rDNA sequences from Stool sample

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Reliance on routinely used taq polymerases for amplification may generate spurious sequences, especially in metagenomic studies utilizing complex mixtures of various DNA templates. Use of high fidelity enzymes and verification of the sequences using various software tools before submission to the...

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Bibliographic Details
Main Author: Vijay Nema
Format: Article
Language:English
Published: Society for the Improvement of Science (SACSIS) 2012-06-01
Series:All Results Journals: Biol
Subjects:
Metagenomics
Sequencing
Chimera;
High fidelity taq polymerase
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Online Access:http://arjournals.com/ojs/index.php?journal=Biol&page=article&op=view&path[]=55&path[]=65

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http://arjournals.com/ojs/index.php?journal=Biol&page=article&op=view&path[]=55&path[]=65

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