A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229E

Abstract Background Currently, one of the most reliable methods for viral infection detection are polymerase chain reaction (PCR) based assays. This process is time and resource heavy, requiring multiple steps of lysis, extraction, purification, and amplification procedures. Herein, we have develope...

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Bibliographic Details
Main Authors: Zachary P. Morehouse, Caleb M. Proctor, Gabriella L. Ryan, Rodney J. Nash
Format: Article
Language:English
Published: BMC 2020-08-01
Series:Virology Journal
Subjects:
PCR
Online Access:http://link.springer.com/article/10.1186/s12985-020-01405-y