MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay.

MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay.

In the present work, we optimised and evaluated a qPCR system integrating 6-FAM (6-carboxyfluorescein)-labelled TaqMan probes and melting analysis using the SYTO 82 (S82) DNA binding dye in a single reaction. We investigated the influence of the S82 on various TaqMan and melting analysis parameters...

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Bibliographic Details
Main Authors: Alexander Nagy, Lenka Černíková, Eliška Vitásková, Vlastimil Křivda, Ádám Dán, Zuzana Dirbáková, Helena Jiřincová, Bohumír Procházka, Kamil Sedlák, Martina Havlíčková
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4816343?pdf=render

Internet

http://europepmc.org/articles/PMC4816343?pdf=render

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