Development of a Quantitative Real-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid

An assay for detecting Apple scar skin viroid (ASSVd) was developed based on nucleic acid sequence based amplification (NASBA) in combination with real-time detection during the amplification process using molecular beacon. The ASSVd specific primers for amplification of the viroid RNA and molecular...

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Bibliographic Details
Main Authors: Seong Heo, Hyun Ran Kim, Hee Jae Lee
Format: Article
Language:English
Published: Hanrimwon Publishing Company 2019-04-01
Series:The Plant Pathology Journal
Subjects:
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6464194