Ethanol causes and lithium prevents neuroapoptosis and suppression of pERK in the infant mouse brain

• Main Authors: Chainllie Young, Megan M.W. Straiko, Stephen A. Johnson, Catherine Creeley, John W. Olney
• Format: Article
• Language: English
• Published: Elsevier 2008-09-01
• Series: Neurobiology of Disease
• Online Access: http://www.sciencedirect.com/science/article/pii/S0969996108001034

Transient exposure of immature animals during the brain growth spurt period to ethanol triggers neuroapoptosis in the developing brain. Here we report that lithium, when administered in a single, well-tolerated dose to infant mice, suppresses spontaneous neuroapoptosis that occurs naturally in the developing brain, and prevents ethanol from triggering neuroapoptosis. To explore lithium's mechanism of action, we focused on kinase signaling systems (ERK, Akt, JNK) that are believed to play a regulatory role in cell survival, and found that very rapidly after ethanol administration there is a suppression of ERK phosphorylation, and that lithium stimulates ERK phosphorylation and prevents ethanol from suppressing this phosphorylation process. Ethanol also suppressed pAKT, but lithium did not counteract this effect. We also found that ethanol activates the JNK system, but this cannot explain the neurotoxic action of ethanol, because JNK activation did not occur in the same neuronal populations that are killed by ethanol.