Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)

Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)

In mammals, interleukin (IL)-4 and IL-13 are important cytokines involved in the immune response of the host defence against pathogens and play critical roles in the polarization of monocytes/macrophages (MO/Mϕs). However, in teleost fish, the role of IL-4/13 homologues in the polarization of MO/Mϕ...

Full description

Bibliographic Details
Main Authors: Xinyu Jiang, Junya Wang, Shuai Wan, Yujie Xue, Zhaosheng Sun, Xingxing Cheng, Qian Gao, Jun Zou
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2020-03-01
Series:Aquaculture and Fisheries
Online Access:http://www.sciencedirect.com/science/article/pii/S2468550X1930190X
id doaj-fc059d64b2e441c38c8abbb3e60abac5
record_format Article
collection DOAJ
language English
format Article
sources DOAJ
author Xinyu Jiang
Junya Wang
Shuai Wan
Yujie Xue
Zhaosheng Sun
Xingxing Cheng
Qian Gao
Jun Zou
spellingShingle Xinyu Jiang
Junya Wang
Shuai Wan
Yujie Xue
Zhaosheng Sun
Xingxing Cheng
Qian Gao
Jun Zou
Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)
Aquaculture and Fisheries
author_facet Xinyu Jiang
Junya Wang
Shuai Wan
Yujie Xue
Zhaosheng Sun
Xingxing Cheng
Qian Gao
Jun Zou
author_sort Xinyu Jiang
title Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)
title_short Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)
title_full Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)
title_fullStr Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)
title_full_unstemmed Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)
title_sort distinct expression profiles and overlapping functions of il-4/13a and il-4/13b in grass carp (ctenopharyngodon idella)
publisher KeAi Communications Co., Ltd.
series Aquaculture and Fisheries
issn 2468-550X
publishDate 2020-03-01
description In mammals, interleukin (IL)-4 and IL-13 are important cytokines involved in the immune response of the host defence against pathogens and play critical roles in the polarization of monocytes/macrophages (MO/Mϕs). However, in teleost fish, the role of IL-4/13 homologues in the polarization of MO/Mϕ remains unknown. In this paper, we identified a second isoform of IL-4/13 (CiIL-4/13A) from grass carp (Ctenopharyngodon idella) in addition to the IL-4/13 homologue previously reported and comparatively studied their expression profiles and functions. The two CiIL-4/13 transcripts were constitutively expressed in tissues but displayed distinct expression patterns. Infection with Flavobacterium columnare or grass carp reovirus II (GCRV-Ⅱ) resulted in the alteration of expression levels of both IL-4/13 isoforms. Furthermore, in the primary head kidney cells, the recombinant CiIL-4/13A and B could up-regulate the expression of IL-10, inducible nitric oxide synthase (iNOS) and M2 macrophage marker gene (KLF4, kruppel like factor 4), whilst down-regulate the expression of IL-1β and M1 macrophage marker gene (MARCO, macrophage receptor with collagenous structure). The results suggest that the CiIL-4/13A and B play similar roles in regulating inflammation and differentiation of MO/Mϕs in grass carp. Keywords: Interleukin 4/13, Cytokine, Macrophage, Bacterial, Grass carp and viral infection
url http://www.sciencedirect.com/science/article/pii/S2468550X1930190X
work_keys_str_mv AT xinyujiang distinctexpressionprofilesandoverlappingfunctionsofil413aandil413bingrasscarpctenopharyngodonidella
AT junyawang distinctexpressionprofilesandoverlappingfunctionsofil413aandil413bingrasscarpctenopharyngodonidella
AT shuaiwan distinctexpressionprofilesandoverlappingfunctionsofil413aandil413bingrasscarpctenopharyngodonidella
AT yujiexue distinctexpressionprofilesandoverlappingfunctionsofil413aandil413bingrasscarpctenopharyngodonidella
AT zhaoshengsun distinctexpressionprofilesandoverlappingfunctionsofil413aandil413bingrasscarpctenopharyngodonidella
AT xingxingcheng distinctexpressionprofilesandoverlappingfunctionsofil413aandil413bingrasscarpctenopharyngodonidella
AT qiangao distinctexpressionprofilesandoverlappingfunctionsofil413aandil413bingrasscarpctenopharyngodonidella
AT junzou distinctexpressionprofilesandoverlappingfunctionsofil413aandil413bingrasscarpctenopharyngodonidella
_version_ 1721570995033079808
spelling doaj-fc059d64b2e441c38c8abbb3e60abac52021-04-02T11:54:27ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2020-03-01527279Distinct expression profiles and overlapping functions of IL-4/13A and IL-4/13B in grass carp (Ctenopharyngodon idella)Xinyu Jiang0Junya Wang1Shuai Wan2Yujie Xue3Zhaosheng Sun4Xingxing Cheng5Qian Gao6Jun Zou7Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Corresponding author. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Corresponding author. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.In mammals, interleukin (IL)-4 and IL-13 are important cytokines involved in the immune response of the host defence against pathogens and play critical roles in the polarization of monocytes/macrophages (MO/Mϕs). However, in teleost fish, the role of IL-4/13 homologues in the polarization of MO/Mϕ remains unknown. In this paper, we identified a second isoform of IL-4/13 (CiIL-4/13A) from grass carp (Ctenopharyngodon idella) in addition to the IL-4/13 homologue previously reported and comparatively studied their expression profiles and functions. The two CiIL-4/13 transcripts were constitutively expressed in tissues but displayed distinct expression patterns. Infection with Flavobacterium columnare or grass carp reovirus II (GCRV-Ⅱ) resulted in the alteration of expression levels of both IL-4/13 isoforms. Furthermore, in the primary head kidney cells, the recombinant CiIL-4/13A and B could up-regulate the expression of IL-10, inducible nitric oxide synthase (iNOS) and M2 macrophage marker gene (KLF4, kruppel like factor 4), whilst down-regulate the expression of IL-1β and M1 macrophage marker gene (MARCO, macrophage receptor with collagenous structure). The results suggest that the CiIL-4/13A and B play similar roles in regulating inflammation and differentiation of MO/Mϕs in grass carp. Keywords: Interleukin 4/13, Cytokine, Macrophage, Bacterial, Grass carp and viral infectionhttp://www.sciencedirect.com/science/article/pii/S2468550X1930190X

Similar Items