An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

We previously reported a CRISPR-mediated knock-in strategy into introns of Drosophila genes, generating an attP-FRT-SA-T2A-GAL4-polyA-3XP3-EGFP-FRT-attP transgenic library for multiple uses (Lee et al., 2018a). The method relied on double stranded DNA (dsDNA) homology donors with ~1 kb homology arms...

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Bibliographic Details
Main Authors: Oguz Kanca, Jonathan Zirin, Jorge Garcia-Marques, Shannon Marie Knight, Donghui Yang-Zhou, Gabriel Amador, Hyunglok Chung, Zhongyuan Zuo, Liwen Ma, Yuchun He, Wen-Wen Lin, Ying Fang, Ming Ge, Shinya Yamamoto, Karen L Schulze, Yanhui Hu, Allan C Spradling, Stephanie E Mohr, Norbert Perrimon, Hugo J Bellen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-11-01
Series:eLife
Subjects:
CRIMIC
CRISPR
drop-in
homologous recombination
drosophila
Online Access:https://elifesciences.org/articles/51539

Internet

https://elifesciences.org/articles/51539

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