A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity

A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity

Gene editing in human embryonic stem cells (hESCs) has been significantly enhanced by the discovery and development of CRISPR Cas9, a programmable nuclease system that can introduce targeted double-stranded breaks. The system relies on the optimal selection of a sgRNA sequence with low off-targets a...

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Bibliographic Details
Main Authors: Camilla Højland Knudsen, Emilía S. Ásgrímsdóttir, Karim Rahimi, Katherine P. Gill, Søs Frandsen, Susanne Hvolbøl Buchholdt, Muwan Chen, Jørgen Kjems, Fabia Febbraro, Mark Denham
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-05-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
CRISPR/Cas9
pluripotent stem cells
INDEL mutation
homologous recombination
human embryonic stem cells
gene targeting techniques
Online Access:http://journal.frontiersin.org/article/10.3389/fcell.2018.00054/full

Internet

http://journal.frontiersin.org/article/10.3389/fcell.2018.00054/full

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