Development and utility of an internal threshold control (ITC) real-time PCR assay for exogenous DNA detection.

Sensitive and specific tests for detecting exogenous DNA molecules are useful for infectious disease diagnosis, gene therapy clinical trial safety, and gene doping surveillance. Taqman real-time PCR using specific sequence probes provides an effective approach to accurately and quantitatively detect...

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Bibliographic Details
Main Authors: Weiyi Ni, Caroline Le Guiner, Philippe Moullier, Richard O Snyder
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3343023?pdf=render