Suspension culture in a T-flask with acoustic flow induced by ultrasonic irradiation
Suspension culture is an essential large-scale cell culture technique for biopharmaceutical development and regenerative medicine. To transition from monolayer culture on the culture surface of a flask to suspension culture in a bioreactor, a pre-specified cell number must first be reached. During t...
Main Authors: | Genichiro Fujii, Yuta Kurashina, Yusuke Terao, Tetsushi Azuma, Akira Morikawa, Kazuhide Kodeki, Osamu Takahara, Kenjiro Takemura |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-05-01
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Series: | Ultrasonics Sonochemistry |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417721000298 |
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