Suspension culture in a T-flask with acoustic flow induced by ultrasonic irradiation

Suspension culture is an essential large-scale cell culture technique for biopharmaceutical development and regenerative medicine. To transition from monolayer culture on the culture surface of a flask to suspension culture in a bioreactor, a pre-specified cell number must first be reached. During t...

Full description

Bibliographic Details
Main Authors: Genichiro Fujii, Yuta Kurashina, Yusuke Terao, Tetsushi Azuma, Akira Morikawa, Kazuhide Kodeki, Osamu Takahara, Kenjiro Takemura
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Ultrasonics Sonochemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1350417721000298