DEVELOPMENT OF THE RECOMBINANT POSITIVE CONTROLS OF BOVINE VIRAL DIARRHEA VIRUS 1 AND 2 FOR PCR ASSAY
Aim. The method of amplifying and cloning the Erns gene of Bovine Viral Diarrhea Virus was developed to obtain the positive controls for polymerase chain reaction. Methods. The strains used in this study were BVDV-1b (Ossloss) and BVDV-2 (Kosice). Viral RNA was extracted by the silica-based extracti...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Odessa I. I. Mechnikov National University
2013-09-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
| Subjects: | |
| Online Access: | http://mbt.onu.edu.ua/article/view/48929 |