Direct sequencing of human gut virome fractions obtained by flow cytometry

The sequence assembly of the human gut virome encounters several difficulties. A high proportion of human and bacterial matches is detected in purified viral samples. Viral DNA extraction results in a low DNA concentration, which does not reach the minimal limit required for sequencing library prepa...

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Bibliographic Details
Main Authors: Mária eDžunková, Giuseppe eD'Auria, Andrés eMoya
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-09-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00955/full