Detection of Viral Pathogens With Multiplex Nanopore MinION Sequencing: Be Careful With Cross-Talk

Detection of Viral Pathogens With Multiplex Nanopore MinION Sequencing: Be Careful With Cross-Talk

Metagenomic sequencing with the Oxford Nanopore MinION sequencer offers potential for point-of-care testing of infectious diseases in clinical settings. To improve cost-effectiveness, multiplexing of several, barcoded samples upon a single flow cell will be required during sequencing. We generated a...

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Bibliographic Details
Main Authors: Yifei Xu, Kuiama Lewandowski, Sheila Lumley, Steven Pullan, Richard Vipond, Miles Carroll, Dona Foster, Philippa C. Matthews, Timothy Peto, Derrick Crook
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-09-01
Series:Frontiers in Microbiology
Subjects:
nanopore sequencing
metagenomics
multiplexing
cross barcode contamination
chimera
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.02225/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2018.02225/full

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