A PVAc-Based Benzophenone-8 Filter as an Alternative to Commercially Available Dichroic Filters for Monitoring Calcium Activity in Live Neurons via Fura-2 AM

In this paper, we design, fabricate, and test a PVAc-based benzophenone-8 (PVAcB-8) emission filter to monitor calcium changes in live neurons. We loaded neurons with fura-2 AM, a calcium-sensitive fluorescent dye, which has dual excitation wavelengths at 340 nm, 380 nm with ratiometric emission at...

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Bibliographic Details
Main Authors: Lior Blockstein, Collin C. Luk, Anil Kumar Mudraboyina, Naweed I. Syed, Orly Yadid-Pecht
Format: Article
Language:English
Published: IEEE 2012-01-01
Series:IEEE Photonics Journal
Subjects:
Online Access:https://ieeexplore.ieee.org/document/6203546/