Two Distinct Approaches for CRISPR-Cas9-Mediated Gene Editing in Cryptococcus neoformans and Related Species
For genetic studies of the Cryptococcus genus, generation of mutant strains is often hampered by a limited number of selectable genetic markers, the tedious process of vector construction, side effects, and other limitations, such as the high cost of acquiring a particle delivery system. CRISPR-Cas9...
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Format: | Article |
Language: | English |
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American Society for Microbiology
2018-06-01
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Series: | mSphere |
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Online Access: | https://doi.org/10.1128/mSphereDirect.00208-18 |