Two Distinct Approaches for CRISPR-Cas9-Mediated Gene Editing in Cryptococcus neoformans and Related Species

For genetic studies of the Cryptococcus genus, generation of mutant strains is often hampered by a limited number of selectable genetic markers, the tedious process of vector construction, side effects, and other limitations, such as the high cost of acquiring a particle delivery system. CRISPR-Cas9...

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Bibliographic Details
Main Author: Ping Wang
Format: Article
Language:English
Published: American Society for Microbiology 2018-06-01
Series:mSphere
Subjects:
Online Access:https://doi.org/10.1128/mSphereDirect.00208-18