A statistical approach for optimizing the protocol for overexpressing lipase KV1 in Escherichia coli: purification and characterization

A statistical approach for optimizing the protocol for overexpressing lipase KV1 in Escherichia coli: purification and characterization

Lipase is one of the most important industrial enzymes, widely used in the preparation of food additives, cosmetics and pharmaceuticals. In order to obtain a large amount of lipase, in the present study, a gene encoding intracellular lipase was cloned from Acinetobacter haemolyticus. The recombinant...

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Bibliographic Details
Main Authors: Kalaivani Batumalaie, Elham Khalili, Naji Arafat Mahat, Fahrul Zaman Huyop, Roswanira Abdul Wahab
Format: Article
Language:English
Published: Taylor & Francis Group 2018-01-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Acinetobacter haemolyticus
central composite design
lipase
protein overexpression
response surface methodology
Online Access:http://dx.doi.org/10.1080/13102818.2017.1407670

Internet

http://dx.doi.org/10.1080/13102818.2017.1407670

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